ABSTRACT
The purpose of this study is to investigate the concentration of plasma choline of Korean and to clarify the relationship between plasma choline concentration and choline intake. Plasma choline concentration of 30 young adults (15 males, 15 females) aged 20 - 30 years living in Deajeon metropolitan city are analyzed and their dietary choline intake. Choline content of one day meal was directly analyzed with the use of enzymatic method. Plasma choline concentration from more than 12 hr fasting blood was analyzed by using HPLC-MS. Choline intakes of male subjects were in the range of 253.51 - 1724.14 mg and those of female subjects were in the range of 240.85 - 938.06 mg. Mean intakes of choline were 634.53+/-353.68 mg in male subjects and 473.99+/-183.76 mg in female subjects. Plasma choline concentration of total subjects was in the range of 5.08 - 14.01 micro mol/L. Mean plasma choline concentration was 9.19+/-2.05 micro mol/L in male subjects and 8.11+/-1.70 micro mol/L in female subjects. Plasma choline concentration did not show significant correlation with choline intake in male and total subjects, but showed positive correlation with choline intake in female subjects (p<0.05). This result shows that more studies on large scaled samples are needed.
Subject(s)
Female , Humans , Male , Young Adult , Choline , Fasting , Meals , PlasmaABSTRACT
BACKGROUND: Hen's egg is the most frequent cause of food hypersensitivity in infants and young children. The major egg white(EW) proteins are ovalbumin(OVA), ovomucoid(OM), ovotransferrine, and lysozyme. OVA and OM have been generally considered to be the most allergic EW proteins. Food irradiation has been permitted in the fields of agriculture and food handling. Currently, it was suggested that the binding ability of specific IgE to gamma-irradiated OVA was rapidly decreased depending upon the irradiation dose. OBJECTIVE: The purpose of this study is to evaluate the application of food irradiation technology as a method for reducing the allergenicities of EW proteins. METHODS: A total of 10 egg-allergic patients less than 24 months of age were enrolled in this study. OVA was gamma-irradiated at 10 kGy in an aqueous state(2.0 mg/mL). Skin prick test and ELISA inhibition test were done to compare the IgE binding capacities before and after irradiation of OVA. RESULTS: One hundred percent of patients showed positive skin reactions with EW and native-OVA. Negative skin reactions to irradiated-OVA were observed in 7 of 10 patients and remainder 3 showed reduced skin reactions(p=0.001). In IgE-ELISA inhibition test, the IgE-binding capacities of irradiated-OVA reduced 1/80 (50% inhibition concentration: native-OVA-0.1 microgram/mL, irradiated-OVA-8 microgram/mL), respectively. CONCLUSION: We suggest that the allergenicity of OVA can be reduced by the treatment with gamma irradiation.